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Comptes Rendus Physique
Volume 5, n° 3
pages 305-313 (avril 2004)
Doi : 10.1016/j.crhy.2004.02.003
Mapping hydrophobic molecular regions using dissolved laser-polarized xenon NMR

Lionel  Dubois,  Patrick  Berthault * ,  J. Gaspard  Huber,  Hervé  Desvaux *
Laboratoire commun de RMN, DSM/DRECAM/service de chimie moléculaire, URA CEA/CNRS 331 Claude Fréjacques, CEA/Saclay, 91191 Gif-sur-Yvette, France 

*Corresponding authors.

Molecular hydrophobic cavities can be mapped thanks to the detection of magnetization transfer from laser polarized xenon to nearby protons. This so called SPINOE approach is described. The study of the spin dynamics during this experiment and its consequences on the practical implementation are detailed. We show that thanks to the knowledge of the physical properties of the system, it becomes possible to choose the best experimental conditions in order to be able to assign magnetization transfer through two dimensional NMR methods. As an illustration, the first 2D SPIROE-TOCSY experiment is reported. To cite this article: L. Dubois et al., C. R. Physique 5 (2004).


Les régions moléculaires hydrophobes peuvent être localisées par l'approche appelée SPINOE qui consiste en la détection des transferts d'aimantation du xénon polarisé par laser dissous vers les protons proches du soluté. Nous rapportons l'étude de la dynamique présente lors de cette expérience et discutons les conséquences sur son implémentation. Nous montrons que la connaissance des propriétés physico-chimiques du système permet de choisir les meilleures conditions expérimentales afin d'être capable d'identifier les transferts d'aimantation via des expériences de RMN à deux dimensions. Nous illustrons ce résultat par le premier spectre de SPIROE-TOCSY. Pour citer cet article : L. Dubois et al., C. R. Physique 5 (2004).

Mots clés  : NMR ; Laser polarized xenon ; SPINOE ; Protein hydrophobic cavity.

Mots clés  : RMN ; Xénon polarisé par laser ; SPINOE ; Cavité hydrophobe de protéines.

© 2004  Académie des sciences@@#104156@@

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